antibodies against serpine2  (R&D Systems)

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).

Article Snippet: Specific primary antibodies against SERPINE2 (Proteintech) was used.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression correlates with tumor immunoregulation, division and proliferation. (A) Volcano map of SERPINE2 co-expressed genes in COAD. (B, C) GO and KEGG enrichment analysis of SERPINE2 co-expressed genes obtained from the cBioPortal, the terms p and q < 0.05 were considered to be significantly enriched. Only 20 leading gene sets are displayed in the plot.

Article Snippet: Specific primary antibodies against SERPINE2 (Proteintech) was used.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is positively correlated with M2 macrophage infiltration in COAD. (A) Scatter plot showing the correlation of 6 different immune cell types and SERPINE2 expression (p < 0.05). The black line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (B) Scatter plot showing the correlation of CD68+ Marcophage and SERPINE2 expression (p < 0.05). C.Scatter plot showing the correlation of M1/M2 macrophage and SERPINE2 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (p < 0.05).

Article Snippet: Specific primary antibodies against SERPINE2 (Proteintech) was used.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is associated with infiltration of CD68 macrophage in COAD patients. (A) The expression of SEPRINE2 in patient tissues was detected by IHC. Adjacent tissue not expressing SERPINE2; tumor tissue strongly expressing SERPINE2. (B) Scatterplot showing the correlation between SERPINE2 and CD68 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the relationship between SERPINE2 and CD68 expression. (C) The expression of SERPINE2 and CD68 in patient tissues were detected by IHC. High levels of SERPINE2 expression are associated with increased expression of CD68. (* p<0.05, **p<0.01, *** p<0.001).

Article Snippet: Specific primary antibodies against SERPINE2 (Proteintech) was used.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: The external secretion of SERPINE2 from colorectal cancer cells promotes macrophage polarization to accelerate the development of colorectal cancer. (A) Schema diagram showing that THP-1 cells were treated with PMA, followed by the treatment of SERPINE2 recombinant protein as indicated treatments to obtain activated macrophages. Then tumor cells cocultured with these macrophages. were used to subsequent experiments. (B, C) RT-qPCR and Westen blot displayed SERPINE2 mRNA and Protein expression were upregulated in colorectal cancer cells. (D) The secretion of SERPINE2 in colorectal cancer cells was detected by ELISA. (E) The effect of SERPINE2 recombinant protein on macrophage polarization. (F) RT-qPCR displayed conditioned medium (CM) derived from colorectal cancer cells promotes the upregulation of macrophage M2 markers, compared to normal Intestinal cells. (G) ELISA analysis demonstrated that SERPINE2 secretion was significantly reduced after SERPINE2 knockdown. (H) RT-qPCR displayed conditioned medium with SERPINE2 knockdown down-regulated macrophage M2 markers. (I, J) The conditioned medium from macrophages treated with SERPINE2 recombinant protein promoted cancer cell proliferation (measured by CCK-8 assay) and migration (assessed by Transwell assay. (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Specific primary antibodies against SERPINE2 (Proteintech) was used.

Techniques: Recombinant, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Knockdown, CCK-8 Assay, Migration, Transwell Assay

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Serum serpin peptidase inhibitor clade E member 2 (SERPINE2) levels were compared between control subjects and patients with type 2 diabetes mellitus (T2DM). a Serum SERPINE2 levels in control subjects and patients with T2DM were detected by enzyme-linked immunosorbent assay (ELISA). The serum SERPINE2 concentration in patients with T2DM ( n = 292) was significantly higher than that in control subjects ( n = 120). Student t test was applied. b Comparison of serum SERPINE2 levels in patients with T2DM with normoalbuminuria, microalbuminuria, and macroalbuminuria. ANOVA was applied. *** P < 0.001

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques: Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Comparison

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Correlation between serum serpin peptidase inhibitor clade E member 2 (SERPINE2) and clinical indicators. Pearson correlation test was performed between SERPINE2 with a estimated glomerular filtration rate (eGFR), b serum creatinine (Scr), c neutrophil gelatinase-associated lipocalin (NGAL), d kidney injury molecule 1 (KIM-1), e transforming growth factor-β1 (TGFβ1), and f connective tissue growth factor (CTGF) in all patients with type 2 diabetes mellitus (T2DM)

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques: Filtration

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve was used to obtain the optimal cutoff value of serum serpin peptidase inhibitor clade E member 2 (SERPINE2) (278.94 pg/mL) that distinguishes the patients with type 2 diabetes mellitus (T2DM) with and without albuminuria. AUC area under the curve

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques:

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Schematic diagram of serpin peptidase inhibitor clade E member 2 (SERPINE2) in the development of diabetic nephropathy. Increased SERPINE2 in patients with type 2 diabetes mellitus (T2DM) with renal dysfunction promotes podocyte injury and albuminuria. SERPINE2 also enhances renal fibrosis by promoting fibrosis-related cytokines, such as transforming growth factor-β1 (TGFβ1) and connective tissue growth factor (CTGF). Therefore, the increase in SERPINE2 levels plays an aggravating role in injury of glomerular and tubular cells caused by hyperglycemia and advanced glycation end products (AGEs)

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques:

Journal: Cancer letters

Article Title: SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

doi: 10.1016/j.canlet.2021.10.001

Figure Lengend Snippet: Fig. 1. SERPINE2 is upregulated in radioresistant NSCLC cells. (A) Schematic illustration of the research idea. (B) Clonogenic assays of A549/A549R and PC9/ PC9R cells treated with the indicated doses. n = 3 wells/group. (C) Tumor growth curves of xenografts of A549 and A549R cells with a single 12-Gy dose (n = 10 mice). (D) Clustered heatmap of RNA-seq data showing the top differentially expressed (up- and downregulated) genes in A549R and A549 cells. The red arrowhead shows the SERPINE2 gene. (E) Quantification of intracellular levels of SERPINE2 and other 16 upregulated proteins in A549R and A549 cells by parallel reaction monitoring (PRM). Red arrowhead indicates the SERPINE2 gene. (F) qRT-PCR analysis of SERPINE2 expression in A549, A549R (left), PC9 and PC9R (right) cells. (G) WB of SERPINE2 and GAPDH in A549, A549R (left), PC9 and PC9R (right) cells. (H) qRT-PCR assessment of SERPINE2 expression in nonirradiated (left) or irradiated (right) A549 and A549R xenograft tumors. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Incubation with primary antibodies against SERPINE2 (Proteintech) was performed at 4 ◦C overnight.

Techniques: RNA Sequencing, Targeted Proteomics, Quantitative RT-PCR, Expressing, Irradiation

Journal: Cancer letters

Article Title: SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

doi: 10.1016/j.canlet.2021.10.001

Figure Lengend Snippet: Fig. 2. SERPINE2 knockdown inhibits migration and invasion and reduces IR-induced G2/M arrest in vitro. (A–B) A wound healing assay showed that SERPINE2 knockdown inhibited the migration of A549R cells. Scale bar: 50 μm. (C–D) Transwell invasion assays demonstrated that SERPINE2 knockdown inhibited the invasion of A549R cells. Scale bar: 20 μm. (E) WB of EMT markers in A549R cells transfected with scrambled shRNA or shSERPINE2. (F) Cell cycle analysis of A549R cells transfected with SERPINE2 shRNA was performed using propidium iodide (PI) staining (48 h after 6-Gy IR). (G) Apoptosis of A549R cells transfected with SERPINE2 shRNA, as detected by annexin V/PI staining by flow cytometry (12 h, 24 h and 48 h after 6-Gy IR). All experiments were performed in at least two independent experiments with three replicate wells. **P < 0.01, ***P < 0.001, n.s., no significance.

Article Snippet: Incubation with primary antibodies against SERPINE2 (Proteintech) was performed at 4 ◦C overnight.

Techniques: Knockdown, Migration, In Vitro, Wound Healing Assay, Transfection, shRNA, Cell Cycle Assay, Staining, Flow Cytometry

Journal: Cancer letters

Article Title: SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

doi: 10.1016/j.canlet.2021.10.001

Figure Lengend Snippet: Fig. 4. SERPINE2 influences the efficiency of HR. (A) Schematic illustration of HR in U2OS-DR-GFP reporter cells. (B) SERPINE2 overexpression results in increased HR efficiency. U2OS-DR-GFP cells were cotransfected with either empty vec tors (negative, Neg) or SERPINE2-FLAG and pCBASceI plasmids. Flow cytometry was conducted to examine the percentage of GFP-positive cells after 48 h. The expression of SERPINE2-FLAG and HA-I-SceI (pCBAS ceI) was verified by WB analysis. (C) Knockdown of SERPINE2 led to decreased HR efficiency. U2OS-DR-GFP cells were transfected with scrambled or SERPINE2 shRNA and then transduced with the pCBASceI plasmid 24 h later. FACS assess ment was applied to determine the percent age of GFP + cells after 48 h. The expression of SERPINE2-FLAG and HA-I-SceI (pCBAS ceI) was verified by WB analysis. (D) Sche matic illustration of NHEJ in U2OS-EJ5-GFP reporter cells. (E–F) Overexpression or knockdown of SERPINE2 did not affect NHEJ efficiency. (G–H) WB analysis of HR-, NHEJ-, and microhomology-mediated end joining (MMEJ)-related genes and SERPINE2 and GAPDH 24 h following 4 Gy of irradia tion in A549 and A549R cells and in A549R cells transduced with scrambled or SER PINE2 shRNA. Relative protein band in tensity is shown below. (I–J) RAD51(green) and DAPI (blue) staining of A549R cells transfected with SERPINE2 shRNA 24 h after 6-Gy IR. Representative images of RAD51 foci are shown in I. We counted the cells with >5 foci as positive. Scale bar: 5 μm *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s., no significance.

Article Snippet: Incubation with primary antibodies against SERPINE2 (Proteintech) was performed at 4 ◦C overnight.

Techniques: Over Expression, Flow Cytometry, Expressing, Knockdown, Transfection, shRNA, Transduction, Plasmid Preparation, Staining

Journal: Cancer letters

Article Title: SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

doi: 10.1016/j.canlet.2021.10.001

Figure Lengend Snippet: Fig. 5. Overexpression of RAD51 restores the radioresistance, radiation-induced DNA damage phenotypes and HR repair efficency of shSERPINE2 cells. (A) WB of RAD51, SERPINE2 and GAPDH in A549R cells stably transfected with SERPINE2 shRNA alone or in combination with RAD51-Myc. (B) Clonogenic survival assays of A549R cells transduced with SERPINE2 shRNA alone or together with RAD51-Myc. n = 4 wells per group. (C) Representative pictures of alkaline comet assays of A549R cells transduced with scrambled shRNA or SERPINE2 shRNA alone or in combination with RAD51-Myc at 24 h after 6-Gy IR. Scale bar: 20 μm. (D) Quantitative assessment of IR-induced DNA damage in C by the tail DNA% and tail moment in the comet assay. (E) RAD51 overexpression increased HR repair efficency of SERPINE2-knockdown cells. **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s.: no significance.

Article Snippet: Incubation with primary antibodies against SERPINE2 (Proteintech) was performed at 4 ◦C overnight.

Techniques: Over Expression, Stable Transfection, Transfection, shRNA, Transduction, Single Cell Gel Electrophoresis, Knockdown

Journal: Cancer letters

Article Title: SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

doi: 10.1016/j.canlet.2021.10.001

Figure Lengend Snippet: Fig. 7. SERPINE2 directly interacts with MRE11. (A) WB analysis of RAD51, p-ATM, ATM, KU80, KU70, SERPINE2 and GAPDH protein levels in A549R cells treated with the MRN complex inhibitor mirin for 48 h. (B–D) Co-IP assays of GFP-tagged SERPINE2 with FLAG-tagged MRE11 (B), RAD50 (C) and NBS1 (D) in 293T cells at 24 h after 4-Gy IR. (E) His pull-down assay of His-tagged SERPINE2 with FLAG-MRE11 (E), RAD50 (F) and NBS1 (G) produced from 293T cells. The ex periments (WB, IP and pulldown assay) were performed in three independent experiments. (H) Schematic representation of SERPINE2 deletion mutants. (I) Co-IP assays of MRE-FLAG with various GFP-tagged full-length SERPINE2 and its deletion mutants in 293T cells.

Article Snippet: Incubation with primary antibodies against SERPINE2 (Proteintech) was performed at 4 ◦C overnight.

Techniques: Co-Immunoprecipitation Assay, Pull Down Assay, Produced

Journal: Cancer letters

Article Title: SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

doi: 10.1016/j.canlet.2021.10.001

Figure Lengend Snippet: Fig. 8. Upregulation of SERPINE2 in LUAD patients correlates with a poor prognosis. (A) Oncomine database analysis revealed upregulation of SERPINE2 mRNA in LUAD tissues versus normal lung tissues. (B–C) Representative histological pictures (B) from IHC staining and the relative IHC scores (C) for SERPINE2 in LUAD tissues (n = 255) and matched adjacent tissues (n = 255). Scale bar: 100 μm. (D–E) OS (D) and RFS (E) were compared between LUAD patients with high or low SERPINE2 expression. (F) OS analysis according to SERPINE2 expression in the LUAD cohort from the Oncomine database. (G) The working model showing how SERPINE2 regulates radiosensitivity and the DDR. For D, E and F, statistical significance was assessed by the log-rank test.

Article Snippet: Incubation with primary antibodies against SERPINE2 (Proteintech) was performed at 4 ◦C overnight.

Techniques: Immunohistochemistry, Expressing

Journal: Frontiers in Pharmacology

Article Title: Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

doi: 10.3389/fphar.2020.566099

Figure Lengend Snippet: qRT-PCR primer sequence of candidate genes associated with metastasis.

Article Snippet: After blocking, membranes were incubated with a primary antibody: rabbit monoclonal against SERPINE2, KRT81, KRT7, DKK1, β-Catenin, WNT3A, NF-kB p65, VEGFC, TNFR1, GAPDH (diluted 1:1,000; ABclonal, China), and mouse monoclonal against N-Cadherin, MMP-9 (diluted 1:1,000; Cell Signaling Technology, USA) for overnight at 4°C.

Techniques: Sequencing

Journal: Frontiers in Pharmacology

Article Title: Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

doi: 10.3389/fphar.2020.566099

Figure Lengend Snippet: DEGs with a consistent trend in both HepG2 and SMMC-7721 cells.

Article Snippet: After blocking, membranes were incubated with a primary antibody: rabbit monoclonal against SERPINE2, KRT81, KRT7, DKK1, β-Catenin, WNT3A, NF-kB p65, VEGFC, TNFR1, GAPDH (diluted 1:1,000; ABclonal, China), and mouse monoclonal against N-Cadherin, MMP-9 (diluted 1:1,000; Cell Signaling Technology, USA) for overnight at 4°C.

Techniques: Membrane, Variant Assay